Protein-protein interactions basically mediate all biological processes. Therefore, understanding these interactions in detail is the main goal of modern biochemistry. Although genomic databases provide us with great opportunities to expand our knowledge of protein-protein interactions, they also bring great challenges to traditional protein chemistry methods. Phage display is a mature combinatorial technology, which is likely to play a greater role in the future of biology. Phage display is an effective and universal method for studying protein-protein interactions. It can be applied to a wide range of protein interaction partners and used in many applications, especially when mapping intracellular interactions of different protein domains. The phage display peptide library is composed of random short peptide sequences of a specific lengths. Short peptide sequences can be obtained through affinity screening of random libraries and target proteins (such as receptors). The sequence analysis and synthesis of the corresponding short peptides, and then we can study the interaction between these two proteins.
Fig 1. The process for selection and affinity maturation of binding peptides from phage-displayed libraries. (Sidhu S S, et al. 2003)
We apply advanced biological analysis platforms and reliable professional knowledge to provide customers with personalized and high-quality services. Our team has extensive experience in phage display-based protein interaction analysis and research and can provide personalized services. Our services are listed below, but not limited to:
Protein-protein interactions usually involve many side chains of each protein. Alanine scanning has been proven to map functional binding epitopes and can be applied to any protein that can be displayed on phage
At Creative Biolabs, our scientists provide phage-derived binding peptide design and culture services for peptidic mimics of extracellular protein binding surfaces.
Although most extracellular protein-protein interactions involve large epitopes formed by discrete regions of the primary sequence, many intracellular interactions depend on the specific recognition of small continuous epitopes within large proteins. We provide phage display peptide library is an ideal tool for analyzing these intracellular peptide binding domains.
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Creative Biolabs can meet the needs of customers by providing protein-protein interaction studies services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
