R408 Helper Phage Production

Helper phages are mutated forms of filamentous phages that replicate their own DNA with minimal efficiency. Its genome has all the functional genes of the M13 phage, but the IG region is defective and cannot be recognized by the pII protein, so the DNA of the helper phage itself cannot perform single-stranded replication. Helper phage can provide pII protein and packaging protein to the phagemid. With the help of helper phage, phagemid can successfully carry out DNA replication and phage packaging to complete the infection of the host. Commonly used helper phages are VCSM13, M13K07 and R408. R408 is an f1 phage mutant with a 24 bp fragment deleted from its IG, which enables it to package single-stranded DNA with an intact signal on its own single-stranded DNA. In addition, it carries a mutation called IRI that makes it insensitive to interference from defective viruses. Unlike M13KO7, R408 does not carry antibiotic resistance marker genes. Superinfection of pEMBL19-containing strain HB101 with helper phage R408 resulted in greatly increased yields of single-stranded plasmid DNA.

Our Services

When wild-type phage is used, interference of the plasmid with phage replication results in a reduction in phage copy number and a dramatic drop in virion yield. Helper phages can overcome interference, maximize virion production and keep the packaging of their own ssDNA low. Our services are listed as follows, but are not limited to:

R408 helper phage production route design
Quality and speciation check
After sales service
R408 helper phage production
R408 helper phage isolation
R408 helper phage purification
R408 helper phage identification
R408 helper phage quantification and reactivity determination by ELISA
R408 helper phage modification

Cysteine modification, N-terminal alanine modification
Aspartic acid and glutamic acid modification
N-terminal serine and threonine modification

Fig 1. Helper phage production

What We Provide

The research results of the biological characteristics of R408 helper phages include the sensitivity test of R408 helper phages under the conditions of ultraviolet light, ether, and chloroform.
We provide helper phage isolation, purification, and proliferation data.
Other information requested by the customer.
Free design assistance from the experts with rich experience and expertise.

Our Advantages

Creative Biolabs has been forming the stereotype of biological science research by discovering, developing, and supporting excellent phage research services.
From the beginning of our company's cultivation, scientific progress and environmental management have been our highest priority.
Our helper phage production service is a high-quality service tailored to customers.
Our helper phage production service is very safe and the isolated helper phages will not affect beneficial bacteria, animal, or human cells.

Creative Biolabs can meet the needs of customers by providing R408 helper phage production services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).