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Rabbit Monoclonal Antibody Library Construction by Phage Display

Rabbit monoclonal antibodies are mainly derived from hybridomas, which are usually not well characterized and sometimes show high batch-to-batch variability. The hybridoma method screening is very limited rare clones are usually missed and it takes a while to produce antibodies (approximately 9 months). In addition, clones are usually very unstable, and the production of antibodies from the hybridoma method can be troublesome. In contrast, phage display combines the superior antigen recognition of the rabbit immune system with the specificity and consistency of monoclonal antibodies produced through hybridoma development without the shortcomings of rabbit hybridomas. Phage display technology screens both phenotype (protein) and genotype (cDNA), so the rabbit monoclonal antibody prepared by this method has more advantages. Additionally, compared with rabbit hybridoma technology, our company's phage display platform can simultaneously produce a larger number of monoclonal antibodies.

Our Services

At Creative Biolabs, the rabbit monoclonal construction service is based on the phage display technology platform, which can provide customers with high-quality services. Rabbit antibody library is a high-quality source of rabbit polyclonal antibodies and monoclonal antibodies. The antibodies produced have the advantages of high specificity, high activity and high affinity.

  • Antigen emulsification
  • Phagemid construction
  • Collect spleen cells and isolate variable region genes
  • Transfection expression
  • Library construction
  • Recloning
  • Antibody screening
  • Ascites preparation
  • Antibody expression
  • Antibody purification

Highlights

Rabbit monoclonal antibodies are a class of reagents with extremely high added value in research, diagnostic and therapeutic fields.

  • Higher affinity (very suitable for IHC use)
  • Higher specificity (higher signal-to-noise ratio)
  • Very suitable for post-translational modification detection

Rabbit mAb Production Service Process

  • Antigen preparation
  • Antigen validation
  • Immunization and serum titer test
  • Library construction & screening
  • Antibody production & purification
  • QC analysis
Workflow of rabbit monoclonal antibody library construction

Our Advantages

  • Faster and cost effective
  • Higher screening range
  • Abundance
  • Enhance the specificity and stability
  • Higher production
  • No fusion
  • Animal protection

Ordering

Ordering workflow of library construction

Creative Biolabs can meet the needs of customers for providing rabbit monoclonal antibody library construction service on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).

Biophage Technology

Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.

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