Rac Prophage RecET Recombination System Construction

Phage recombination systems have been widely used in biotechnology to modify prokaryotic species, to create transgenic animals and plants, and, more recently, to genetically manipulate human cells. The RecET recombination system was found to be a mutation in E. coli recB and recC strains that suppresses the host recombination defect of these mutants. This mutation, called sbcA (recBC repression), upregulates the expression of a recombinant system from an endogenous cryptic prophage of E. coli called Rac. When used to promote binding recombination in E. coli, this pathway is called the RecE pathway and requires many components of the RecA and RecF pathways. For plasmid recombination in E. coli, the RecE pathway is independent of RecA. The Rac phage recombination system is encoded by two genes, recE and recT, which are functionally similar to l exo and l bet, respectively. It is shown that the RecET system can replace l Red for growth and recombination in recA-E. coli hosts.

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Research report shows that RecET recombination reaction in E. coli can be used for efficient gene cloning. The RecET recombination system provides an efficient tool for directly targeting genes in bacterial chromosomes. We provide construction services of Rac prophage RecET recombination system construction services. They are listed as follows, but are not limited to:

• Expression of Rac prophage RecET recombination genes
• Rac prophage RecET recombineering design
• Rac prophage RecET recombineering construction
• Efficiency determination of the RecET recombination system

Key Components of the P22 Recombineering System

The Rac phage recombination system is encoded by two genes, recE and recT, which are functionally similar to l exo and l bet, respectively.

• RecT: RecT is a ssDNA annealing protein that is structurally and functionally similar to l Beta protein. RecT has been shown to promote joint molecule formation by linearly pairing circular ssDNA with homologous dsDNA and facilitates strand transfer from linear duplexes to circular ssDNA.
• RecE: The RecE protein is a 50-30 dsDNA exonuclease that uses a progressive digestion mode and is therefore very similar to l Exo. The effect of the RecET protein on dsDNA ends is thought to be conceptually similar to that of the Red protein in that RecE generates 30 ssDNA tails that are subsequently bound by the RecT single-stranded DNA annealing protein (SSAP).

Workflows

Creative Biolabs can meet the needs of customers by providing construction services of Rac prophage RecET recombination system on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).