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Rat Antibody Library Construction by Phage Display

Rat Antibody Library Construction by Phage DisplayAntibodies are widely used in drug development. They can be used as an important reference for immunogenicity analysis, and can specifically detect the level of antibody drugs in the body. Therefore, antibodies are important reagents for pharmacokinetic research. The use of laboratory rats can be traced back to the 18th century when a group of domestic albino brown rats was established for the first time for a series of pure scientific purposes. Since the publication of the fully sequenced rat genome, this species has become one of the most important and widely used experimental animals, second only to its rodent brother, the mouse. As a global brand focusing on the technology, products and services of phage research and development companies, Creative Biolabs is committed to providing high-quality target antigens and key reagents and related services for the development of targeted therapeutic drugs. Creative Biolabs provides a series of rat antibodies with high affinity, high specificity and high sensitivity. At the same time, in order to meet the diverse needs of customers, Creative Biolabs also provides one-stop development services from rat antibodies, pharmacokinetics to immunogenicity testing.

Our Services

Creative Biolabs proposes the construction of an immune library, in scFv format, focused on the target of interest combined with high throughput screening by phage display. This optimizes the chances of success in obtaining high affinity and high specificity antibodies. Our services are listed as follows, but are not limited to:

  • Rat antibody library design
  • Rat antibody library construction by phage display
  • Magnetic bead high-throughput screening
  • Antibody pairing (optional)
  • Functional verification (optional): ELISA, WB
  • Ascites preparation
  • Antibody expression
  • Antibody purification
  • QC analysis

Rat Monoclonal Antibody Development Workflow

  • Standard antibody production service evaluation
  • Discussion before production: customized specifications, flow charts, costs
  • Purchase order
  • Production specification approval
  • Monoclonal antibody research and development progress report

Antigen Requirement

  • Protein Immunization: 500 μg for two rats
  • Peptide Immunization: conjugated-peptide (1mg) for immunization and free-peptide (0.5mg) for screening step
  • DNA Immunization: plasmid containing DNA target-of-interest

Library Building and Phage Display

  • Selection of a large number of scFv candidates
  • Optimized screening with different stringency conditions (direct, competitive, subtractive, etc. )
  • Immediate and automatic access to sequences (single clones - redundancy analysis)
  • Production, purification, and characterization of candidates (reactivity, affinity, etc. )
  • At the end of this phase, the client will have the opportunity to test the scFv and select the best candidates for the reformatting phase

Benefits of Rat Monoclonal Antibodies

Rat Antibody Library Construction by Phage Display
  • Monoclonal antibodies with higher sensitivity
  • Antibodies with a higher affinity to the antigen
  • Excellent results, especially for small and weak immunogenic antigens
  • Additional epitope recognition
  • Proximity to lymph nodes and unique immune response
  • Rat antibody Fc is very close to human antibody
  • Rat antibodies are very suitable for mouse antigens

Creative Biolabs can meet the needs of customers by providing phage display library construction services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).

Biophage Technology

Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.

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