Recombinant antibodies are monoclonal antibodies produced in vitro using synthetic genes. Unlike conventional monoclonal antibodies, recombinant antibodies are prepared without animals. Instead, they are produced by detecting the gene sequence of the antibody; then inserting the gene into an appropriate cell line to produce the antibody. Because the antibody is positive, even if the original cell line dies or mutates, the desired cell line can be generated through gene insertion. This ensures the high specificity and stability of the recombinant antibody. In addition, compared with conventional antibodies, recombinant antibodies can provide a higher level of batch-to-batch consistency. Creative Biolabs has been focusing on the development and production of recombinant antibodies for more than 3 years. In addition to the advantages of recombinant antibodies, Creative Biolabs' recombinant monoclonal antibodies are also known for their higher purity and affinity, as well as minimal batch-to-batch deviation. We can guarantee that we produce and verify the quality of each of our antibodies in-house.
Fig 1. Different antibody formats used for phage display. (Anand T, et al. 2021)
Recombinant antibodies, also known as genetically engineered antibodies, refer to the use of recombinant DNA and protein engineering techniques to modify or recombine antibody genes according to different needs and express them in appropriate recipient cells after transfection. Creative Biolabs can provide customers with one-stop recombinant antibody production services according to their different needs.
This stage includes animal immunization with the desired antigen, then separation of B lymphocytes, mRNA extraction and cDNA synthesis.
Genes related to different clones of antibodies are digested with restriction enzymes, cloned into a phagemid vector, and then displayed on the surface of the phage.
After cloning the fragments into the phagemid vector, display various antibody clones on the surface of the phage.
Our scientists use different methods for screening for antibody screening, such as immunoassay, immunocytochemistry, active separation of cells due to their fluorescent properties and immunoblotting.
Creative Biolabs has been focusing on applying advanced phage display technology to a wide range of service projects, especially the development of recombinant antibodies.
Creative Biolabs provides various types of recombinant antibodies, including full-length antibodies, scFv, Fab, sdAb, isotype/subtype and various forms of Fc fusion proteins.
Creative Biolabs' recombinant antibodies have been verified on multiple platforms, including WB, ELISA and Neutralising.
Creative Biolabs can meet the needs of customers by providing recombinant antibody production services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
