Single-chain antibody (scFv) is a recombinant protein formed by splicing the variable region genes of antibody heavy and light chains through a linker gene (linker) encoding about 5-25 AA. scFv has the advantages of small molecular weight, strong tissue penetration, short circulation half-life in vivo, low immunogenicity, and easy genetic engineering improvement. It plays an important role in clinical diagnosis, treatment, and prevention of diseases. Using phage display technology, scFv antibodies with high affinity and stable structure can be prepared. People use phage display technology to prepare scFv phage antibody library, and the phage antibody screened by the phage antibody library usually has high stability. The pCANTAB 5E phage display system is the most commonly used system for scfv phage surface display. The system consists of phagemid vector (pCANTAB 5E), host bacteria (E. coli TG1, HB2151, etc.), helper phage M13K07 and other parts. The host bacteria in the pCANTAB 5E system are E. coli TG1 and E. coli HB2151. Phagemids infect the two host bacteria to obtain two different products. Infection of E. coli TG1 can finally obtain the scfv-plll fusion protein. Escherichia coli HB2151 can finally obtain soluble scFv protein.
We have been raising industry standards to provide scFv phage display system design and construction services in accordance with customer needs. According to strict quality assurance and biosynthetic quality control standards, we increase reliability and improve the workflow of laboratory technicians. Our services are listed as follows, but are not limited to:
Phage display technology is used to construct the scFv phage display system, and the phage antibodies screened by the phage display system usually have high stability. The pCANTAB 5E phage display system is the most commonly used system for scFv phage surface display. The system consists of a phagemid vector (pCANTAB 5E), host bacteria (E. coli TG1, HB2151, etc. ), helper phage M13K07, and other parts.
Creative Biolabs can meet the needs of customers by providing phage display system construction on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
