The key difference between serine and tyrosine recombinases is that in serine recombinases, serine is the nucleophilic amino acid that the enzyme uses to attack DNA during site-specific recombination whereas in tyrosine recombinases, tyrosine Acids are nucleophilic amino acids that enzymes use to attack DNA during site-specific recombination of DNA. Tyrosine recombinases are structurally diverse and functionally diverse, including integrases, soluble phenolases, invertases, and transposases. Recent studies have shown that the serine recombinase family is equally versatile, with members in a variety of structural forms. Prototype cleavage/convertases are highly regulated, affecting only resolution or inversion, and they have an N-terminal catalytic domain and a C-terminal DNA-binding domain. Phage-encoded serine recombinases, such as the φC31 integrase, cause integration and excision with tightly controlled directionality, and have an N-terminal catalytic domain but a C-terminal domain in contrast to dissociable resolase/invertases longer. These observations suggest that serine recombinases are modular and that fusion of catalytic domains to unrelated sequences creates structural and functional diversity.
Fig 1. Domain structures of serine recombinases (Stark W M, 2014)
Creative Biolabs scientists can use a variety of production strategies to help researchers gain insight into serine recombinase production. Our team helps you achieve greater success by providing high-quality service. Our services are listed below, but are not limited to:
Our serine recombinases are derived from Streptomyces, Mycobacterium, Listeria monocytogenes, Lactobacillus, Enterococcus faecalis and Bacillus cereus.
Creative Biolabs can meet the needs of customers by providing production services of phage recombinases on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
