Subtracted cDNA Library Construction

For some experiments, a complete cDNA library is not necessary, on the contrary, a subtracted cDNA library is useful. The subtracted cDNA library contains cDNA clones corresponding to mRNAs that are present in one cell or tissue type but not in the second type. The cDNA library is used to isolate a set of cDNA clones corresponding to a type of mRNA, or to help isolate cDNA clones corresponding to specific mRNAs. The screening procedure of cDNA clones is laborious, because specific DNA or antibody probes are not available. Creative Biolabs adopts the SMART library construction method to form cDNA on the resistance network, connect the vector with the target, and transform the host for gene research and development.

Our Services

High-quality subtracted cDNA libraries are used for the identification of differentially expressed genes. Creative Biolabs will provide you with cDNA library construction services and provide detailed test reports. Our services are listed as follows, but are not limited to:

Total RNA isolation
Extraction RNA from a variety of samples.
Synthesis full-length cDNA
Library construction
Harvest primary clones
Meshing of 1000 points on nylon membrane
Data Analysis

Features

Confirm subtraction at each step by northern, Q-RT PCR, colony hybridization, and/or high-throughput sequencing
Mirror direction selection (MOS) eliminates false positive clones from the SSH library
Virtual Northern Blot
Each sample receives two size fractionation libraries (0.4 kb-2.0 kb and 2.0 kb and above)
90-100% recombination efficiency
5 million primary clones
Phage resistance competent cell used as a breeding host
250-500 µg endotoxin-free transfection grade DNA is provided with the library
Semi-solid amplification procedure is suitable for amplification to prevent biased clone performance

Our Advantages

Depth and breadth of knowledge
Specialist expertise
Global delivery

Our Capabilities

No PCR-based subtraction (results from PCR amplification and restriction enzyme digestion techniques)
To non-specific amplification and smaller insertions)
Quality control via virtual Northern blot analysis, library screening and PCR
Average insert size ~1.8 kb
Fast turnaround time
Complete package

Creative Biolabs can meet the needs of customers for providing subtracted cDNA library construction services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).