The tailed phage (Caudovius) has a larger genome, the genome size of such phages is 16-735 kb, and the number of genes carried ranges from a few to hundreds. The core genes of tailed bacteriophages include 1) structural protein gene module, capsid protein gene, tail silk protein gene, tail sheath protein gene (base plate), collar protein gene (neck), etc.; 2) DNA replication, repair, and packaging gene modules, DNA polymerase genes, terminate genes, DNA ligase genes, DNA helicase, and exonuclease genes, etc.; 3) Gene transcription and translation gene modules, RNA polymerase gene, tRNA, etc. These characteristics of tailed phage genomes make it possible to design and synthesize artificial phages modularly. In the modular design of phage genes, considering the time sequence of gene expression, the promoter, ribosome binding site (RBS), and terminator of the corresponding genes must also be moved simultaneously according to the sequence of early, middle, and late genes. Synthetic phages can also be endowed with new properties by integrating other functional genes, such as biofilm depolymerization genes, in regions between different gene functional modules.
Synthetic biology has been applied to improve phage infection efficiency, improve phage biosafety, alter phage host range, tune bacterial communities, and knock out specific bacterial genes. Developments in synthetic biology will inspire our teams to design modular phages as multifunctional biologics for clearing multidrug-resistant bacteria, detecting pathogens, modulating bacterial diversity, and drug delivery.
Remove some non-essential genes and add genetic elements, or modularly design the entire phage genome, and split the designed genome sequence into Oligo sequences of about 100 nt that are conducive to synthesis
Poor or no expression, truncated proteins, misincorporation of amino acids are the most common consequences of codon bias. This question has been extensively studied for E. coli expression.
Sanger sequencing targets specific regions of template DNA using oligonucleotide sequencing primers that bind to adjacent regions of DNA.
We offer next-generation sequencing (NGS) for phage gene sequencing, a massively parallel sequencing technology that provides ultra-high throughput, scalability and speed.
Creative Biolabs provides proprietary technologies and complementary services to organizations involved in the development of synthetic phage projects.
Creative Biolabs can meet the needs of customers by providing synthetic phage genome design services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
