Direct chemical or electrotransformation of synthetic phage genomes into host cells for rescue requires high transformation efficiency, especially for large phage genomes. Although efficient transformation protocols have been devised for some bacteria, such as Escherichia coli and Pseudomonas aeruginosa, many other bacterial species are extremely difficult to transform and produce viable phage particles. This becomes a limiting bottleneck for in vitro or yeast-based synthesis of phage genomes. Currently, cell-free expression systems and L-form cell expression provide potential solutions to this problem. L-form bacteria are bacteria that have been engineered or artificially selected to not have a cell wall. When polyethylene glycol is present, cells are very fragile but readily take up foreign DNA. The cell-free system is an efficient and rapid in vitro protein synthesis method, that is, on the basis of cell extracts, exogenous supplementary components (e.g. RNA polymerase, amino acids, tRNA,) are added, and DNA is synthesized in vitro. Transcribed into RNA and translated into protein. Through the continuous optimization of the cell-free expression system, it can be used for high-throughput expression of proteins and validation of in vitro gene circuits.
The synthetic phage genome, after rescuing the active phage particles, our researchers mainly detect its one-step growth curve, antibacterial curve, and morphology, and further identify the function of the synthesized phage. Our services are listed as follows, but are not limited to:
Cell-free systems are an in vitro tool that is widely used to study biological responses that occur within cells in addition to intact cellular systems, thereby reducing the complex interactions typically found when working in whole cells.
Creative Biolabs can meet the needs of customers by providing synthetic phage genome synthesis services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
