Synthetic Phage Genome Synthesis

Our team synthesized short Oligo fragments (100 nt) in vitro according to the designed phage genome sequence and then assembled by PCR amplification, Gibson assembly, or yeast recombination platform to form a complete phage genome. In recent years, the technology of artificially designing and synthesizing large fragment phage genomes has matured, which can be divided into two ways: in vivo assembly and in vitro assembly. In vitro assembly is to carry out phage gene recombination assembly by PCR, restriction enzyme ligation, Gibson assembly, and Golden Gate and other methods, and then transform it into host bacteria for phage genome rescue. The genomes of some bacteriophages, such as filamentous bacteriophage M13 or RNA bacteriophage MS2, are small enough (<10 kb) to be easily assembled in vitro. However, the genomes of most tailed phages are too large (>20 kb) to be easily manipulated in vitro, and when amplifying phage gene fragments in bacteria, bacterial growth may be inhibited. many researchers have explored the assembly of phage genomes in vivo. Yeast readily ingests exogenous DNA and possesses a powerful homologous recombination system, making yeast a platform for efficient splicing of large DNA fragments while avoiding the cytotoxicity associated with phage genes.

Our Services

Advances in synthetic biology have greatly promoted the artificial synthesis of bacteriophage genomes, making it feasible and convenient for us to design and synthesize bacteriophage de novo. Our services are listed as follows, but are not limited to:

• Sequence design and codon optimization
• Comprehensive de novo gene synthesis at your request
• Standard sequence synthesis from 0.5 to 5 kb
• Standard cloning into high copy number, AmpR or KanR, pUC derived plasmids
• Double-stranded sequencing data for each gene
• Affordable synthetic phage production services
• Efficient site-directed mutagenesis of your provided genes (cDNA, ...)

What We Offer?

• Creative Biolabs provides proprietary technology and complementary services to organizations involved in phage development.
• Creative Biolabs works with companies and academic groups around the world, including most specialized biological companies, to support the development and manufacture of better phage synthesis methods for researchers.
• Our bioassay group offers a range of standardized phage-based sequencing and stability tests that can be customized to your specific requirements. Since biology programs are rarely standard, we also offer custom assay development services.

Creative Biolabs can meet the needs of customers by providing synthetic phage genome synthesis services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).