T4 Phage Display System Construction

T4 phage display system is a new display system established in the mid-1990s. It is worth noting that it can fuse two exogenous peptides or proteins with completely different properties with the coat proteins smaller outer capsid (SOC, 9 ku) and highly immunogenic outer capsid (HOC, 40 ku) on the surface of the T4 capsid, and directly on the surface of the T4 phage Display. Therefore, the expressed protein does not require complicated protein purification, which avoids protein denaturation and loss caused by purification. Because HOC and SOC are non-essential accessory proteins in the structure and life history of phage, their presence or absence does not affect the survival and reproduction of T4, so HOC and SOC have become the key group that can be genetically manipulated in the T4 phage display technology system. One or two exogenous polypeptides (proteins) can be fused to the C-terminus of SOC and/or fused to the N-terminus of HOC and displayed on the surface of T4 phage, but it does not affect the normal activity of T4 phage.

Fig 1. (A) Major structural components of bacteriophage T4. (B) A schematic of the phage T4 infection process. (Maghsoodi A, et al. 2019)

Our Services

T4 Phage display is an exponentially growing field of research. It has had a major impact on immunology, cell biology, drug discovery, and pharmacology, and is becoming increasingly important in plant science. Our services are listed as follows, but are not limited to:

SOC site phage display system construction
HOC site phage display construction
Dual display of SOC site and HOC site construction

T4 Phage Display System Application

Cancer therapy
Vaccine design
Enzyme analysis, enzyme inhibition
Nucleic acid-protein interaction
Epitope mapping
Mutation analysis
Antibody development
Diagnostic assays
Drug discovery

Key Features of T4 Phage Display System

Larger insert
Higher display density
Both N-end and C-end insertion are available
Multiple choices of host strains besides E. coli

Our Advantages

International standards
Flexibility
Custom services
Global delivery

Creative Biolabs can meet the needs of customers for providing T4 phage display system construction services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.

Reference

Maghsoodi A, Chatterjee A, Andricioaei I, et al. How the phage T4 injection machinery works including energetics, forces, and dynamic pathway[J]. Proceedings of the National Academy of Sciences, 2019, 116(50): 25097-25105.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).