T7 phage is an icosahedral virus of the Podoviridae family, with a linear double-stranded (ds) DNA genome. Similar to T4, phage T7 has a head and tail structure. The icosahedral head of T7 conserves its dsDNA genome is composed of 415 copies of capsid gp10, arranged as 60 hexamers on the surface and 11 pentamers at the apex. The main coat protein gp10 has two isotypes, gp10A and gp10B, with a ratio of 9 to 1, caused by a natural translation frameshift at amino acid 341. The minor protein gp10B is caused by a frameshift at the end of the gene, making the capsid protein 52 residues longer. The fusion protein is displayed on the 52 extra residues C-terminal protein gp10B. In this way, it can avoid problems related to steric hindrance. T7 phage particles exhibit high stability under various extreme conditions, including high temperature and low pH, which contribute to effective high-throughput affinity elutriation.
Fig 1. Detailed structure of T7 phage. (Deng X, et al. 2018)
Creative Biolabs provides high quality T7 phage display system construction services. Our experts have a high level of knowledge and experience in phage display. Our services are listed as follows but are not limited to:
The latest application of T7 phage display system helps to reveal the mechanism of molecular interaction, it is used in many fields, such as:
Creative Biolabs can meet the needs of customers for providing T7 phage display system construction services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
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