Tyrosine recombinases (YRs) form a large family of proteins that carry out site-specific DNA recombination in various biological processes. They promote post-replicative segregation of plasmids and circular chromosomes during cell division, thereby protecting genome integrity in bacteria, archaea and bacteriophages. For example, the highly conserved Xer proteins (e.g. XerC and XerD in E. coli) resolve chromosomal multimers formed after prokaryotic DNA replication, and Cre recombinase separates dimers from the P1 phage genome. Other YRs act as genetic switches, triggering phenotypic variation within bacterial populations through DNA inversions or deletions. Tyrosine recombinase breaks single strands of DNA and forms covalent 3' - phosphotyrosine bonds, promoting reversible recombination between two identical sites. Tyrosine recombinase has very strict requirements on the core sequence of the recognition site. YRs contribute to the emergence and spread of bacterial virulence and antibiotic resistance in multiple ways, and characterizing their distribution and function contributes to understanding horizontal gene transfer and adaptation in bacteria.
Fig 1. Structural homology of tyrosine recombinases. (Meinke G, et al. 2016)
Tyrosine recombinases contribute to the emergence and spread of bacterial virulence and antibiotic resistance in multiple ways, and characterizing their distribution and function contributes to understanding horizontal gene transfer and adaptation in bacteria. Our researchers have developed a recombination phage platform technology that can be easily and precisely engineered for specific phage recombinases applications. Our services are listed as follows, but are not limited to:
Creative Biolabs can meet the needs of customers by providing production services of phage recombinases on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
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