The replication and packaging machinery provided by helper phages acts not only on the phagemid genome but also on the genome of the helper phage itself. During the screening, those recombinant phages containing helper phage genomes may non-specifically bind to the selected target molecules, which seriously reduces the screening efficiency. Therefore, helper phages are very important for the quality of phagemid libraries. There are two main types of helper phages constructed by different mechanisms: full-length ll-containing helper phages, gl-deleted, or glI-deficient helper phages. The helper phages containing full-length glII mainly include M13KO7, R408, and VCSM13, which are the most widely used helper phages and are commonly referred to as standard helper phages. VCSM13 is a mutant of M13KO7. To propagate (amplify) the VCSM13 helper phage, E. coli TG1, ER2738, or strain XL1-Blue MRF' is required, as those strains contain the supE44 mutation that provides a glutamine suppressor tRNA. The VCSM13 helper phage is strictly used to generate single-stranded DNA from excised phagemids.
VCSM13 is commonly used in phage display. After VCSM13 helper phage production and purification, the typical quality controls will be performed by our scientists. we provide helper phage identification services include mass spectrometry (MS), absence of contaminating phages, titer determination by double agar layer method (DAL), time-kill assays, qPCR or ELISA. Our services are listed below, but are not limited to:
Creative Biolabs can meet the needs of customers by providing CM13 helper phage production on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
