Recombineering, a recently developed technique for efficient genetic manipulation of bacteria, is facilitated by phage-derived recombinant proteins and has the advantage of using DNA substrates with short regions of homology. The system was originally developed in E. coli but has since been adapted for other bacteria. It is now widely used in many different systems for a variety of purposes, as well as for the construction of chromosomal gene knockouts, deletions, insertions, point mutations, and in vivo cloning, mutagenesis, and construction of bacterial artificial chromosomes and plasmids. Genomic libraries have been reported. However, these methods can also be effectively applied to the genetic modification of phage genomes, including phage and lytic growth states. The growing collection of fully sequenced phages raises more questions than they answer, including the unknown functions of a large number of genes with no known homologues and unknown functions. Recombination of phage genomes is at the heart of solving these problems, enabling the simple construction of mutants, the determination of gene necessity, and the elucidation of gene functions.
Fig 1. E. coli phage recombineering. (Marinelli, L. J. et al, 2012)
Phage recombination systems have been widely used in biotechnology for modifying prokaryotic species, creating transgenic plants and animals, and more recently for genetic manipulation of human cells. We provide construction services of phage recombination system construction services. They are listed as follows, but are not limited to:
Phage recombinases have been used as valuable genetic tools and novel bacteriophage enzymes, and their potential applications in genetic engineering and genome manipulation, vector science, and generation of new transgene delivery vectors, and cell therapy are attractive areas of research, need to continue research.
Creative Biolabs can meet the needs of customers by providing construction services of phage recombination system on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
