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Phage Display cDNA Library Construction

Phage Display cDNA Library Construction

cDNA library is based on a specific tissue or cell mRNA as a template, and then through reverse transcription of the mRNA to form cDNA. And it is ligated with an appropriate vector and transformed into the recipient bacteria to form a recombinant DNA clone group. In this way, the cDNA clone collection containing all the mRNA information of the cell is called cDNA. cDNA library specifically reflects the protein-coding gene expressed in a certain tissue or cell at a specific developmental stage, so the cDNA library has tissue or cell specificity. For cDNA phage display libraries, Creative Biolabs uses a new approach that avoids restriction enzyme-mediated cDNA truncation, just as other vendors usually use for cDNA cloning. Therefore, our scheme improves the chance of displaying full-length proteins on the surface of phage. Creative Biolabs also constructs standard, subtracted and full-length cDNA libraries in different phage display vectors.

Our Services

Creative Biolabs provides faster, cheaper and better cDNA library construction services. Our professional design team will ensure that you get a series of cDNA library construction services you need, including standard cDNA library, subtracted cDNA library, standardized cDNA library, full-length cDNA library, etc. Our services are listed as follows, but are not limited to:

  • Standard cDNA Library
    The cDNA library is produced using our proprietary technology, which has full-length enriched, high-quality size fractionation and directional cloning of cDNA.
  • Subtracted cDNA Library
    A high-quality subtracted cDNA library for the identification of differentially expressed genes.
  • Normalized cDNA Library
    The standardization process removes redundant and multiple replicated genes, such as ribosomes and other house-keeping genes. This leads to the same representation of low-copy genes and high-copy genes in the library.
  • Full-length cDNA Library
    The full-length cDNA library is constructed by chaining full-length mRNA, and then synthesizing the first and second.

cDNA Library Construction Platform

cDNA libraries are constructed in the following systems:

Purification Strategy

  • T7 Select series of vectors
  • M13 based phagemid vectors
  • Yeast display (YD) vectors
  • Ribosome display systems

Our Capabilities

Phage Display cDNA Library Construction
  • No need for large amounts of RNA. Only a few hundred nanograms of total RNA or mRNA is sufficient to construct a library
  • Directed library generation
  • Does not digest cDNA, because we do not use EcoRI/HindIII in the cloning process
  • Express larger cDNA on the surface of phage
  • Higher clonal diversity
  • Deliver at least 10 million primary clones

Creative Biolabs can meet the needs of customers for providing phage display cDNA library construction services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).

Biophage Technology

Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.

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