Phage Display Peptide Library Construction

Phage display is a powerful tool that can be used to select binding partners from a complex library. The technology utilizes the direct connection between phage-displayed peptides or proteins and the nucleic acid encoding them. Among the large number of peptide or protein variants displayed in the phage library (10⁹ - 10¹⁰), peptides or protein variants with high affinity and specificity to the target are separated by repeated affinity selection. Finally, the sequence of the positive binding variant is determined by sequencing the gene encoding the displayed peptide or protein. In recent years, phage display peptide libraries have made significant progress. A variety of biologically active molecules, including antibodies, receptors, and enzymes, have selected peptide ligands with high affinity and/or high specificity from many different types of peptide libraries. Based on years of expertise in this field, we can use our peptide library construction technology to advance your project. We can also use our experience to build a custom library and design a selection and screening strategy that best suits your specific project.

Fig. 1. Biopanning selection of phage-displayed peptide libraries using serum samples¹.

Our Services

Our phage display peptide library is a collection of highly complex libraries in linear and Cys-constrained format. They also include conformational peptide libraries to support any requirements. Our services are listed as follows, but are not limited to:

• Linear random peptide libraries construction
• Cys-constrained random peptide libraries construction
• Peptide libraries screening
• Peptide libraries sequencing
• Peptide libraries analysis
• Phage display combinatorial peptide library construction
• Phage displayed cyclic-peptide library construction
• Phage display homing peptide library construction
• Phage display Cys-constrained random peptide library construction

Our Capabilities

• In-house libraries:
• Linear and Cys-constrained random peptide library in M13 pVIII and pIII display formats
• Linear and Cys-constrained conformational peptide library based on HC-CDR3 in M13 pVIII and pIII display formats
• Select screening design according to specific project requirements:
• In vivo/intracellular recombinant protein
• Screening by monoclonal analysis and/or NGS
• Binding and/or functional determination

Our Advantages

Service Features

• No cross-contamination: Lyophilize peptides directly in a 96-well plate or tube rack to avoid cross-contamination.
• Comprehensive peptide modification services: Our modification services include labeling peptides, coupling and using disulfide bonds to cyclize peptides.
• Ready-to-use delivery results: Our researchers put the peptide library in a 96-well plate or tube rack and directly delivers it to the customer. After receiving it, it can be directly used for subsequent experiments.
• Quality control: MS and HPLC identification results are provided for each peptide product.

Feedback to Customer

• Freeze-dried peptides in wells
• Polypeptide position table
• Identification result

Creative Biolabs can meet the needs of customers for providing phage display peptide library construction services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.

Reference

• Wu C H, Liu I J, Lu R M, et al. Advancement and applications of peptide phage display technology in biomedical science[J]. Journal of biomedical science, 2016, 23(1): 1-14.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).