Phage Display Scaffold Library Construction

There are abundant sources of protein scaffolds in nature. The choices of appropriate protein scaffolds and screening methods of the display can advance the construction of optimized restricted random libraries based on reasonable protein skeleton structures. Compared with non-restricted random libraries, it is expected to obtain a protein structure with a new function or a ligand molecule with a higher affinity for the target molecule. At present, the random peptide library restricted by backbone proteins has shown great potential values in high-efficiency target molecule screening, basic research, clinical diagnosis and medical treatment. At present, most protein scaffolds use small proteins or protein domains in natural proteins, such as bacterial receptors, DNA-binding proteins, and protease inhibitors. Phage display allows the concept of small protein scaffolds to be combined with the design of random protein libraries, where the affinity function is displayed in the accessible surface area of the scaffold.

Our Services

Creative Biolabs provides engineered protein scaffold library construction services. Our services have offered a solid foundation for the successful isolation and development of high-affinity binders in the fields of research, diagnosis and treatment. Our services are listed as follows, but are not limited to:

• Engineered protein scaffold library construction with phage display system
• Scaffold library design for phage display screening
• Affilin ready-to-panning phage display library construction
• GFP ready-to-panning phage display library construction
• Darpins ready-to-panning phage display library construction

Other Related Services

• Engineered protein scaffold library construction with mRNA display system
• Engineered protein scaffold library construction with ribosome display system
• Engineered protein scaffold library construction with cell surface display system
• Engineered protein scaffold library construction with ribosome display system

Our Capabilities

Creative Biolabs can provide customers with the construction services of backbone protein libraries based on the following proteins.

Classification of Miniprotein Scaffolds

According to the peptide backbone, small protein scaffolds can be divided into three categories:

• The first type of scaffold shows a single exposed ring embedded in the ridge template.
• The second type of scaffold contains a discontinuous surface area formed by secondary structure elements.
• The third category shows a complex backbone architecture, which contains multiple discontinuous variable loop segments to form a coherent interface.

Fig 1. Schematic representations of different scaffold categories. (Zoller F, et al., 2011）

Reference

• Zoller F, Haberkorn U, Mier W. Miniproteins as phage display-scaffolds for clinical applications[J]. Molecules, 2011, 16(3): 2467-2485.

Creative Biolabs can meet the needs of customers for providing engineered protein scaffold library construction services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).