Before studying any bacteriophage or using its biological properties, it must first be separated and purified. Therefore, in many explorations of phage biology and biotechnology, separation and purification are the key steps. For a phage to be used for phage therapy, it must be isolated from the environment and exhibit certain characteristics, not just a strain that kills the target bacterial pathogen. These include desirable characteristics, such as a relatively broad host range and lack of other characteristics, as well as the ability to carry toxin genes and form lysogens. Although bacteriophages are usually isolated first and then characterized, the isolation procedure can be changed to bias the isolation towards phages with the desired characteristics. There are several classical and modern techniques to isolate bacteriophages, and the selection of the appropriate method usually depends on the intended use of the bacteriophages. At Creative Biolabs, our team can provide the state of art technologies to separate and purify phages, not only samples obtained from the environment but also, we pay special attention to the separation and purification of therapeutic phages.
Our scientists have extensive experience in phage isolation and purification. Our team can develop and validate isolated methods for phage projects. Our services are listed as follows, but are not limited to:
| Method | Description | Advantages |
|---|---|---|
| Spot testing | The plate is inoculated with host bacteria to form a lawn, and then small drops of phage filtrate are placed on the surface. The lysis zone after incubation indicates the presence of phage. | It is simple and allows multiple phage filtrates to be tested on the same plate. |
| Plaque testing | The increasingly diluted phage filtrate is mixed with the bacteria and placed on the surface of the plate by spreading or covering with soft agar. | It can prove effective phage growth. |
| Culture lysis | The phage filtrate is added to the broth culture of the bacteria and incubated. | it is useful for bacteria that will not grow to confluence on solid media. |
| Routine test dilution | The phage lysate is diluted to the extent that it produces less than confluent lysis on the plate. | It is suitable for phages that do not form obvious plaques or very small fuzzy plaques. |
Creative Biolabs can meet the needs of customers by providing phage isolation and purification services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
