Phage purification is obtained by inoculating a phage suspension with a double agar method and a susceptible host strain and further purifying the phage contained in the plaque. The double agar method is to mix a small amount of diluted phage suspension and host cells in melted "soft" agar. The resulting suspension is then poured on an appropriate "nutrient" alkaline agar medium to form a thin "top layer" that hardens and fixes the bacteria. During the culture process, uninfected bacteria multiply to form fusion bacteria, and the bacteria grow on the surface of the petri dish. Each infected bacterium will explode within a short period of time and release offspring phages that infect neighboring bacteria, and these phages are lysed again. The goal of phage purification is to ensure that your final phage sample contains only one type of phage. In other words, once purified, all phage particles in the sample should be genetic clones of each other. This is called the "homogeneous population" or "clonal population" of bacteriophages.
Bacterial and phage inoculum, nutrient type, agitation, oxidation, pH, and temperature have a great influence on the purity of phage. With extensive experience in phage purification, our experts provide a viable phage production system according to the international guidelines of Good Manufacturing Practice (GMP). Our featured purification services are listed as follows, but are not limited to:
It is recommended to perform three rounds of purification to obtain a pure lysate of each phage. There are two ways to purify dental plaque:
Some phages degrade the host's DNA but do not consistently reach a safe level. Proteases should be removed because they will negatively affect the shelf life of the phage. The purification process is designed to consistently meet the specifications of the target phage. Our typical quality control methods are listed as follows:
We will include your phage in the phage database for use by other scientists
Creative Biolabs can meet the needs of customers for providing phage purification services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
