Phage Display Antibody Library Construction

Phage display antibody library is one of the most prominent researches in antibody engineering. This technology mainly displays the antibody on the surface of phage, and uses the target antibody molecule to express the antibody molecule in the field according to the library sequence. According to the source of the library sequence, there are four types of phage display antibody libraries: naïve, immune, semi-synthetic and synthetic libraries. Creative Biolabs can construct a complete library with high and possible difficult monoclonal antibodies from multiple types such as human lamb, camel method, rabbit, rabbit, rabbit, cow and so on. In comparison, the new antibody phage display library has obvious advantages in stealth monoclonal antibodies and even fully humanized antibodies.

Our Services

The phage display technology service platform currently developed by Creative Biolabs covers a variety of antibody library types, and can customize personalized libraries according to the special needs of customers, and provide flexible and efficient screening services. Our services are listed as follows, but are not limited to:

• Naïve library construction
• Immune library construction
• Semi-synthetic library construction
• Synthetic library construction
• Library screening and biopanning
• ELISA screening
• Assessment of the affinity of single phage binders
• DNA extraction and antibody sequencing

Antibody Production Technologies

For the production of monoclonal antibodies, hybridomas and phage display are both very important technologies. However, each method has its limitations:

Purification Strategy

• Affinity tag: 6xHis tag.
• Molecular format: Insert SUMO between the His6 tag and the protein of interest (POI).
• Purification: IMAC.
• Remove tags after purification: Cut the C-terminal of SUMO with SUMO protease.

Workflow

Our Advantages

• Automated phage-displayed high-throughput screening to achieve rapid delivery
• Natural antibody library capacity: up to 10¹⁰, immune antibody library capacity: >10⁷
• Using affinity detection technology to select the best humanized framework region sequence, rapid and high-throughput screening of high-affinity and high-yield humanized antibodies
• Fragment insertion rate > 90%

Ordering

Creative Biolabs can meet the needs of customers for providing phage display antibody library construction services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).