Although research on phages as anti-infective agents has progressed slowly, phages have played an important role in the development of molecular biology. In recent years, the increase in multidrug-resistant bacteria has revived interest in the use of bacteriophages as antibacterial agents. With the wide range of possibilities offered by genetic engineering, these bacterial viruses are being modified to precisely control and detect bacteria and serve as a source of new antimicrobial agents. In applications beyond their antibacterial activity, phages have also been developed as vehicles for drug delivery and vaccines, as well as for the assembly of new materials. Phage genome editing is crucial for uncovering the molecular mechanisms of viral infection and designing phages with enhanced antibacterial properties. Phage genome engineering has opened the way to study the role of viral genes in infection and to generate phages with novel properties. Phage genetic engineering relies primarily on homologous recombination (HR) to aid in the targeted elimination of wild-type phages by CRISPR-Cas nucleases.
Creative Biolabs is a leading provider of safety testing for phage development programs, offering Sanger sequencing, next-generation DNA sequencing, real-time PCR, digital PCR, Southern blotting, Northern blotting, and other molecular biology and microbiology methods to support our customers' need. Our services are listed as follows, but are not limited to:
Virulent phage genomes are usually edited by homologous recombination (allelic exchange), first, DNA edited is integrated into a plasmid and transformed into the host bacterium.
Bacteriophage recombineering of electroporated DNA (BRED) is for the simple and efficient construction of targeted phage mutants.
In vivo recombineering, phages are used as engineering tools for other less-studied E. coli phages.
CRISPR-Cas-mediated bacteriophage genome engineering avoids scooping out a very small percentage of recombinant phage from a large number of wild-type phages.
Creative Biolabs provide a whole-gene synthesis platform for specifying the optimal solution for bacteriophage whole-genome synthesis and assembly from synthetic oligonucleotides.
Phage genomes assembled, modified, and propagated in yeast have been isolated and introduced into bacteria to generate functional phage particles.
Cell-free transcription-translation systems can generate phage or virus-like particles from DNA in vitro, thereby overcoming the need for a highly competent bacterial host.
Creative Biolabs can meet the needs of customers by providing phage genome editing services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
