Phage display technology was first proposed by George Smith in 1985. It is used as an expression vector, capable of presenting foreign amino acid sequences that can bind to antibodies. Since then, a large number of phage display peptide and protein libraries have been constructed, leading to various techniques for screening such libraries. This technology has had a significant impact on work and discovery in the fields of immunology, cell biology, pharmacology, and drug discovery. Phage display allows large libraries of peptides and proteins to be displayed on the surface of filamentous phage, thereby selecting peptides and proteins with high affinity and specificity for almost any target, including antibodies. Phage display libraries containing up to 1010 variants can be constructed simultaneously. Phage particles can withstand very harsh conditions, such as low pH and low temperatures, without losing bacterial infectivity. Therefore, a protocol using low pH and high urea concentration has been used to separate bound phage from the target. In addition, the bound phages do not need to be eluted from the micropores or animal tissues before bacterial infection. Conversely, after adding bacteria directly to the wells or homogenized organs or tissues, the infection can continue.
Phage display is a rapidly developing technology. According to the nature of the phage display library, it has been used to support a series of applications. These include epitope mapping, receptor and ligand identification, protein-protein interaction studies, recombinant antibody production, directed evolution of proteins, and drug discovery. Our services are listed as follows:
Creative Biolabs can provide high-quality custom services on phage display platform according to their different needs. Generally speaking, phage display technology has the following 5 steps:
Recombinant DNA technology is used to integrate foreign cDNA into viral DNA.
These libraries are exposed to selected targets, and only some phages will interact with the targets. The goal is to plan to identify specific ligands, such as immobilized proteins, cell surface proteins, or vascular endothelium.
Unbound phages can be washed away, leaving only those phages that show affinity for the receptor.
The target-bound phage is recovered by elution.
The eluted phage showing specificity is used to infect new host cells for amplification, or direct bacterial infection and amplification of recovered phage.
Creative Biolabs can meet the needs of customers by providing custom services based on phage display on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
