Bacteriophage is a virus that can infect and replicate within bacteria and archaea in order to inject its genome into its cytoplasm. Bacteriophages are composed of proteins that encapsulate DNA or RNA genomes and may have relatively simple or complex structures. Phage display was first developed by G. Smith in 1985 as a method to present peptides on the surface of lysogenic filamentous phage. In phage display technology, the gene encoding the target protein is inserted into the phage coat protein gene, so that the phage displays the protein outside. And protein contains genes inside, leading to the link between genotype and phenotype. In practice, the protein or peptide to be displayed is usually expressed as a fusion with the phage coat protein pIII or pVIII. This type of fusion protein is directed to the bacterial periplasm or intracellular membrane through an appropriate signal sequence added to its N-terminus. The new requirements and challenges of various applications continue to promote its improvement and update, and display technology systems based on bacteriophages M13, λ, T4 and T7 have appeared one after another.
Phage display is a seemingly simple technology but difficult to do. With years of experience, we can provide phage display services, such as high-quality phage display library construction and customized phage display library screening services to meet your various needs accurately. Our phage display systems construction services are listed as follows:
A filamentous bacteriophage is a type of bacteriophage, defined by its filamentous or rod-like shape. Filamentous bacteriophages usually contain single-stranded DNA genomes and infect Gram-negative bacteria. Ff, M13, fd and f1 families are important phages that can be used for phage display, among which M13 phage is the most commonly used.
λ phage is a bacterial virus that infects the bacterial species E. coli.
T4 phage is a phage that infects E. coli. It is a member of the T-even bacteriophage, which includes enterobacteriophages T2 and T6. The T4 phage is a relatively large phage, approximately 90 nm wide and 200 nm long. Its double-stranded DNA genome is about 169 kbps, encoding 289 proteins.
T7 bacteriophage is an icosahedral virus of Podoviridae family, with a linear double-stranded (ds) DNA genome.
Creative Biolabs can meet the needs of customers for providing phage display system construction services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
