The replication and packaging machinery provided by helper phages acts not only on the phagemid genome but also on the genome of the helper phage itself. During the screening, those recombinant phages containing helper phage genomes may non-specifically bind to the selected target molecules, which seriously reduces the screening efficiency. Therefore, helper phages are very important for the quality of phagemid libraries. CM13 is a helper phage specially designed for phage display. Phage display is a powerful method for the evolution of proteins and peptides with new functions, but the properties of the molecules that can be evolved are limited by the encoded chemical diversity. CM13 is a derivative of M13KO7 containing an anti-interfering ir3B A->G mutation at position 8418 of M13KO7. CM13 is equivalent to the VCSM13 helper phage and can be directly replaced. CM13-infected cells generally produced more virions than M13KO7, while displaying similar levels of the minor coat protein III. This helper phage makes the small-scale production of virions particularly efficient, thereby facilitating the entire screening process.
Creative Biolabs' helper phage production platform supports different types of phage production. Our team is composed of experienced senior biologists, who have accumulated rich experience in the process of production method development, and they continue to promote the study of helper phage for higher standards. Our services are listed below, but are not limited to:
Creative Biolabs can meet the needs of customers by providing CM13 helper phage production on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
